Difference between revisions of "Part:BBa K4438811"

 
Line 6: Line 6:
  
  
−
<!-- Add more about the biology of this part here
+
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
The part MN4_trigger_6_midtrigger_phi29(BBa_K4438811) has complementarity to the part Aptamer_MN4 (BBa_K4438800) and disrupts its three-way junction.
 +
Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. Quinine binds with cocaine-binding aptamer MN4 [1] and displaces the MN4_trigger_6_midtrigger_phi29(BBa_K4438811) . This part has complete complementarity with MN4_Target_6(BBa_K4438812). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers.
 +
Different levels of quinine can be detected using all these three parts.
  
 
<!-- -->
 
<!-- -->
Line 18: Line 21:
 
<partinfo>BBa_K4438811 parameters</partinfo>
 
<partinfo>BBa_K4438811 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
===References===
 +
Reinstein, O., Yoo, M., Han, C., Palmo, T., Beckham, S. A., Wilce, M. C., & Johnson, P. E. (2013). Quinine binding by the cocaine-binding aptamer. Thermodynamic and hydrodynamic analysis of high-affinity binding of an off-target ligand. Biochemistry, 52(48), 8652-8662.

Revision as of 12:27, 12 October 2022


MN4_trigger_6_midtrigger_phi29

MN4_trigger_6_midtrigger_phi29(BBa_K4438811) is single-stranded DNA having 10 nucleotides. Figure 1B) shows the secondary structure and minimum free energy. The sequence is complementary to the middle region of the Aptamer_MN4 (BBa_K4438800) with no mismatched bases.


Usage and Biology

The part MN4_trigger_6_midtrigger_phi29(BBa_K4438811) has complementarity to the part Aptamer_MN4 (BBa_K4438800) and disrupts its three-way junction. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. Quinine binds with cocaine-binding aptamer MN4 [1] and displaces the MN4_trigger_6_midtrigger_phi29(BBa_K4438811) . This part has complete complementarity with MN4_Target_6(BBa_K4438812). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. Different levels of quinine can be detected using all these three parts.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Reinstein, O., Yoo, M., Han, C., Palmo, T., Beckham, S. A., Wilce, M. C., & Johnson, P. E. (2013). Quinine binding by the cocaine-binding aptamer. Thermodynamic and hydrodynamic analysis of high-affinity binding of an off-target ligand. Biochemistry, 52(48), 8652-8662.