Difference between revisions of "Part:BBa K243000"

(→‎Sequence specific nuclease)
(→‎Sequence specific nuclease)
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===Sequence specific nuclease===
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===Sequence specific nuclease(1968)===
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The researchers H.O. Smith, K.W.
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Wilcox, and T.J. Kelley (Johns Hopkins University 1968), were the
 +
first persons who isolated and characterized the first restriction
 +
nuclease whose functioning depended on a specific DNA nucleotide
 +
sequence. This was a big breakthrough for the genetic engineering, it
 +
gave the scientists a tool for working with the DNA. Now over forty
 +
years later over 3000 restriction enzymes have been studied in
 +
detail, and more than 600 of these are available commercially and are
 +
routinely used for DNA modification and manipulation in laboratories.
  
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This important development came when H.O. Smith, K.W. Wilcox, and T.J. Kelley, working at Johns Hopkins University in 1968, isolated and characterized the first restriction nuclease whose functioning depended on a specific DNA nucleotide sequence.
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:24, 14 October 2009

Protein domain (active) of the restriction endonuclease FokI

This part is used as the active domain of our universal restriction endonuclease. It cut DNA, when it fused with the inactive protein domain of our universal restriction endonuclease(BBa_K243001)and linked with specific oligos.


Sequence specific nuclease(1968)

The researchers H.O. Smith, K.W. Wilcox, and T.J. Kelley (Johns Hopkins University 1968), were the first persons who isolated and characterized the first restriction nuclease whose functioning depended on a specific DNA nucleotide sequence. This was a big breakthrough for the genetic engineering, it gave the scientists a tool for working with the DNA. Now over forty years later over 3000 restriction enzymes have been studied in detail, and more than 600 of these are available commercially and are routinely used for DNA modification and manipulation in laboratories.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 487