Difference between revisions of "Part:BBa K243000"
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===Sequence specific nuclease=== | ===Sequence specific nuclease=== | ||
| − | This important development came when H.O. Smith, K.W. Wilcox, and T.J. Kelley, working at Johns Hopkins University in 1968, isolated and characterized the first restriction nuclease | + | This important development came when H.O. Smith, K.W. Wilcox, and T.J. Kelley, working at Johns Hopkins University in 1968, isolated and characterized the first restriction nuclease whose functioning depended on a specific DNA nucleotide sequence. |
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Revision as of 12:06, 14 October 2009
Protein domain (active) of the restriction endonuclease FokI
This part is used as the active domain of our universal restriction endonuclease. It cut DNA, when it fused with the inactive protein domain of our universal restriction endonuclease(BBa_K243001)and linked with specific oligos.
Sequence specific nuclease
This important development came when H.O. Smith, K.W. Wilcox, and T.J. Kelley, working at Johns Hopkins University in 1968, isolated and characterized the first restriction nuclease whose functioning depended on a specific DNA nucleotide sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 487