Difference between revisions of "Part:BBa K4283012"

 
 
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<partinfo>BBa_K4283012 short</partinfo>
 
<partinfo>BBa_K4283012 short</partinfo>
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<p>OMV released was controlled using the SulAp promoter (BBa_K518010), an optogenetic regulatory element that was previously used by our team in 2020.</p>
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<b>Figure 1 </b>
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[[File:fig 3012 1.png|400px|thumb|center|Design of light control OMV release]]
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<b>Figure 2 </b>
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[[File:fig 3012 2.png|400px|thumb|center|UV control OMV producing vector pSB1C3_sulAp_ClyA-Adpgk-eGFP]]
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<p>After transforming the vector into E.coli Top10 the bacteria were exposed to UV radiation (15mW/CM2) for 1min and tested for change of the florescence. </p>
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<b>Figure 3 </b>
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[[File:fig 3012 3.png|400px|thumb|center|Change in Florescence of bacteria culture]]
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<p>The florescence of bacteria culture increases significantly after exposure to UV. It was decided to perform further testing through western blot. </p>
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<b>Figure 4 </b>
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[[File:fig 3012 4.png|400px|thumb|center|Western blot image of bacteria after UV radiation]]
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More information on:[https://2022.igem.wiki/sz-shd/engineering Engineering Success | SZ-SHD - iGEM 2022]
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OMV released was controlled using the SulAp promoter (BBa_K518010), an optogenetic regulatory element that was previously used by our team in 2020.
 
  
 
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K4283012 SequenceAndFeatures</partinfo>
 
  
  

Latest revision as of 14:16, 12 October 2022


UV controlled OMV release

OMV released was controlled using the SulAp promoter (BBa_K518010), an optogenetic regulatory element that was previously used by our team in 2020.

Figure 1

Design of light control OMV release

Figure 2

UV control OMV producing vector pSB1C3_sulAp_ClyA-Adpgk-eGFP

After transforming the vector into E.coli Top10 the bacteria were exposed to UV radiation (15mW/CM2) for 1min and tested for change of the florescence.

Figure 3

Change in Florescence of bacteria culture

The florescence of bacteria culture increases significantly after exposure to UV. It was decided to perform further testing through western blot.

Figure 4

Western blot image of bacteria after UV radiation

More information on:Engineering Success | SZ-SHD - iGEM 2022