Difference between revisions of "Part:BBa K174012"
 
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<partinfo>BBa_K174012 parameters</partinfo>
 
<partinfo>BBa_K174012 parameters</partinfo>
 
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===References===
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#Moriyama, R., A. Hattori, S. Miyata, S. Kudoh, and S. Makino. 1996. A gene (sleB) encoding a spore cortex-lytic enzyme from Bacillus subtilis and response of the enzyme to l-alanine-mediated germination. J. Bacteriol. 178:6059-6063

Latest revision as of 22:40, 20 October 2009

sleB, Bacillus subtilis germination gene with RBS

sleB encodes for a spore cortex-lytic enzyme. This enzyme responds germination triggered by L-alanine which is the most universal germinant for spores. It has been shown that B. subtilis mutants with inactivated sleB gene were not able to germinate (1).

Hence by regulating this gene in sleB mutant B. subtilis cells, germination rate can be contolled.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Moriyama, R., A. Hattori, S. Miyata, S. Kudoh, and S. Makino. 1996. A gene (sleB) encoding a spore cortex-lytic enzyme from Bacillus subtilis and response of the enzyme to l-alanine-mediated germination. J. Bacteriol. 178:6059-6063