Difference between revisions of "Part:BBa K188634"

Revision as of 21:39, 31 October 2009

RBS+mRFP+double Terminator

This device is part of device F(BBa_K188661) in our project without ccdB gene(BBa_K145151) and promoter lacI/pL(BBa_R0011). We expect that the AHL-luxR complex would bind to promoter lacI/pL(BBa_R0011) and express the the ccdB gene for E. coli suicide when project is done. And express mRFP to indicate the the project is done.

So this device only can be used to add a specific functional promoter(and other target gene), and measure the activity of the promoter by measure the expression of mRFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 573
Illegal AgeI site found at 685
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K188634 short</partinfo>
-RBS + highly engineered mutant of red fluorescent protein from Discosoma striata (coral) + double terminator
+This device is part of device F(BBa_K188661) in our project without ccdB gene(BBa_K145151) and promoter lacI/pL(BBa_R0011). We expect that the AHL-luxR complex would bind to promoter lacI/pL(BBa_R0011) and express the the ccdB gene for E. coli suicide when project is done. And express mRFP to indicate the the project is done.
+So this device only can be used to add a specific functional promoter(and other target gene), and measure the activity of the promoter by measure the expression of mRFP.
+[[Image:dd.jpg]]
 <!-- Add more about the biology of this part here