Difference between revisions of "Part:BBa K4438708"
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P4G13_Target_4 (BBa_K4438708) is a single-stranded DNA sequence having 91 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 5’ end has a few bases complementary to the P4G13_trigger_4_phi29(BBa_K4438707). The middle region has ssDNA sense T7 promoter sequence. The 3’ end has an ssDNA sense broccoli sequence.  
 
P4G13_Target_4 (BBa_K4438708) is a single-stranded DNA sequence having 91 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 5’ end has a few bases complementary to the P4G13_trigger_4_phi29(BBa_K4438707). The middle region has ssDNA sense T7 promoter sequence. The 3’ end has an ssDNA sense broccoli sequence.  

Revision as of 12:02, 12 October 2022

P4G13_Target_4

P4G13_Target_4 (BBa_K4438708) is a single-stranded DNA sequence having 91 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 5’ end has a few bases complementary to the P4G13_trigger_4_phi29(BBa_K4438707). The middle region has ssDNA sense T7 promoter sequence. The 3’ end has an ssDNA sense broccoli sequence.


Usage and Biology

This part along with P4G13_trigger_4_phi29 (BBa_K4438707) and P4G13_aptamer (BBa_K4438700) can be used for sensing different concentrations of progesterone [1].



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Contreras Jiménez, G., Eissa, S., Ng, A., Alhadrami, H., Zourob, M., & Siaj, M. (2015). Aptamer-based label-free impedimetric biosensor for detection of progesterone. Analytical chemistry, 87(2), 1075-1082.