Difference between revisions of "Part:BBa K4438702"
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<partinfo>BBa_K4438702 short</partinfo> | <partinfo>BBa_K4438702 short</partinfo> | ||
| − | P4G13_Target_1 (BBa_K4438702) is a single-stranded DNA sequence having 81 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 3’ end has few bases complementary to P4G13_trigger_1_w/oPol (BBa_K4438701). In the middle complete ssDNA antisense T7 promoter sequence is present. The 5’ end has broccoli cDNA sequence. | + | P4G13_Target_1 (<partsinfo>BBa_K4438702</partsinfo>) is a single-stranded DNA sequence having 81 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 3’ end has few bases complementary to P4G13_trigger_1_w/oPol (<partsinfo>BBa_K4438701</partsinfo>). In the middle complete ssDNA antisense T7 promoter sequence is present. The 5’ end has broccoli cDNA sequence. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This part along with P4G13_trigger_1_w/oPol (BBa_K4438701) and P4G13_aptamer (BBa_K4438700) can be used for sensing different concentrations of progesterone [1]. | + | This part along with P4G13_trigger_1_w/oPol (<partsinfo>BBa_K4438701</partsinfo>) and P4G13_aptamer (<partsinfo>BBa_K4438700</partsinfo>) can be used for sensing different concentrations of progesterone [1]. |
| − | + | https://parts.igem.org/File:T--IISER-Tirupati_India--Pg413_1.png | |
| − | + | ||
Revision as of 13:21, 12 October 2022
P4G13_Target_1
P4G13_Target_1 (<partsinfo>BBa_K4438702</partsinfo>) is a single-stranded DNA sequence having 81 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 3’ end has few bases complementary to P4G13_trigger_1_w/oPol (<partsinfo>BBa_K4438701</partsinfo>). In the middle complete ssDNA antisense T7 promoter sequence is present. The 5’ end has broccoli cDNA sequence.
Usage and Biology
This part along with P4G13_trigger_1_w/oPol (<partsinfo>BBa_K4438701</partsinfo>) and P4G13_aptamer (<partsinfo>BBa_K4438700</partsinfo>) can be used for sensing different concentrations of progesterone [1]. https://parts.igem.org/File:T--IISER-Tirupati_India--Pg413_1.png
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Contreras Jiménez, G., Eissa, S., Ng, A., Alhadrami, H., Zourob, M., & Siaj, M. (2015). Aptamer-based label-free impedimetric biosensor for detection of progesterone. Analytical chemistry, 87(2), 1075-1082.