Difference between revisions of "Part:BBa K4390092"
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<partinfo>BBa_K4390092 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4390092 SequenceAndFeatures</partinfo>
  
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==Reference==
  
 
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Revision as of 00:11, 14 October 2022


His-tagged WT RolA

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

Usage and Biology

The wild-type RolA with 6His-tag for Nickel affinity column purification.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 169
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 161

Reference