Difference between revisions of "Part:BBa K4255102"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K4255102 short</partinfo> | ||
+ | |||
+ | We divided the seven enzymes into two parts, 4CL, CHS and CHI catalyzed the production of naringenin one by one, and naringenin was further generated into anthocyanins in the presence of F3H, F3'5'H, DFR and ANS. The F3H, F3'5'H, DFR and ANS. | ||
+ | <!-- sequences come from (哪四个物种). --> | ||
+ | We constructed the last four enzymes on the pRSFDuet vector, in which F3H was fused to F3'5'H and DFR was fused to ANS. | ||
+ | The function of the enzymes can be listed below: | ||
+ | F3H: catalyze flavanone into dihydrokaempferol | ||
+ | F3’5’H: catalyze dihydrokaempferol into dihydromyricetin | ||
+ | DFR: catalyze dihydromyricetin into delphinin colorless | ||
+ | ANS: catalyze delphinin colorless into delphinidin | ||
+ | |||
+ | |||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K4255102 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K4255102 parameters</partinfo> | ||
+ | <!-- --> |
Revision as of 11:04, 12 October 2022
pRSFDuet-F3H, F3'5'H-DFR, ANS
We divided the seven enzymes into two parts, 4CL, CHS and CHI catalyzed the production of naringenin one by one, and naringenin was further generated into anthocyanins in the presence of F3H, F3'5'H, DFR and ANS. The F3H, F3'5'H, DFR and ANS.
We constructed the last four enzymes on the pRSFDuet vector, in which F3H was fused to F3'5'H and DFR was fused to ANS.
The function of the enzymes can be listed below:
F3H: catalyze flavanone into dihydrokaempferol
F3’5’H: catalyze dihydrokaempferol into dihydromyricetin
DFR: catalyze dihydromyricetin into delphinin colorless
ANS: catalyze delphinin colorless into delphinidin
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]