Difference between revisions of "Part:BBa K4119003"
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<partinfo>BBa_K4119003 short</partinfo>
 
<partinfo>BBa_K4119003 short</partinfo>
  
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it's Pvgb-Bs2-Cpa fdx terminator
+
In this part of our project, the key promoter vgb was a microaerobic induced promoter of Vitreoscilla hemoglobin gene. Considering the gene compatibility difference between different host bacteria, we designed the pMTL-Pvgb-bs2 plasmid to determine whether the promoter vgb could work normally in Clostridium tyrobutyricum by detecting the fluorescent expression intensity of fluorescent protein Bs2.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:09, 12 October 2022


Pvgb-Bs2-Cpa fdx terminator

In this part of our project, the key promoter vgb was a microaerobic induced promoter of Vitreoscilla hemoglobin gene. Considering the gene compatibility difference between different host bacteria, we designed the pMTL-Pvgb-bs2 plasmid to determine whether the promoter vgb could work normally in Clostridium tyrobutyricum by detecting the fluorescent expression intensity of fluorescent protein Bs2.


Document

Results:

The test group: the fluorescence intensity is relatively high

The control group: the fluorescence intensity is lower compared to the test group

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 350
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 350
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 350
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 350
  • 1000
    COMPATIBLE WITH RFC[1000]