Difference between revisions of "Part:BBa K4255101"
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We constructed the first three enzymes on the pETDUET vector, in which 4CL was linked to CHS via linker to form a fusion protein and CHI was expressed independently. The function of the enzymes can be listed below: 4CL: catalyze phenylalanine into 4-coumaryl coenzyme A CHS: catalyze 4-coumaryl coenzyme A into chalcone CHI: catalyze chalcone into flavanone | We constructed the first three enzymes on the pETDUET vector, in which 4CL was linked to CHS via linker to form a fusion protein and CHI was expressed independently. The function of the enzymes can be listed below: 4CL: catalyze phenylalanine into 4-coumaryl coenzyme A CHS: catalyze 4-coumaryl coenzyme A into chalcone CHI: catalyze chalcone into flavanone | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | ===Other Information=== | ||
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Revision as of 10:48, 12 October 2022
pETDuet-gm4CL-gmCHS+gmCHI
We constructed the first three enzymes on the pETDUET vector, in which 4CL was linked to CHS via linker to form a fusion protein and CHI was expressed independently. The function of the enzymes can be listed below: 4CL: catalyze phenylalanine into 4-coumaryl coenzyme A CHS: catalyze 4-coumaryl coenzyme A into chalcone CHI: catalyze chalcone into flavanone
Usage and Biology
Other Information
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3580
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]