Difference between revisions of "Part:BBa K174011:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | In ''B. subtilis'', out of five kinases only KinA, KinB and KinC kinases are effective to trigger the sporulation. KinD and KinE do not | + | In ''B. subtilis'', out of five kinases only KinA, KinB and KinC kinases are effective to trigger the sporulation. KinD and KinE do not phosphorylate Spo0F, hence do not effect the sporulation. We chose KinA for our device. |
As our whole system grew, it was not possible to test everything. Rather than LacI which can be derepressed by IPTG, we had another transcription factor that represses the device's promoter. Instead, we designed our promoter with flanking sites to be induced by IPTG and unit test it. The idea was to construct everything and plug them all together after we test each component. | As our whole system grew, it was not possible to test everything. Rather than LacI which can be derepressed by IPTG, we had another transcription factor that represses the device's promoter. Instead, we designed our promoter with flanking sites to be induced by IPTG and unit test it. The idea was to construct everything and plug them all together after we test each component. | ||
Revision as of 18:31, 13 October 2009
IPTG inducable kinA Bacillus sporulation trigger
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In B. subtilis, out of five kinases only KinA, KinB and KinC kinases are effective to trigger the sporulation. KinD and KinE do not phosphorylate Spo0F, hence do not effect the sporulation. We chose KinA for our device.
As our whole system grew, it was not possible to test everything. Rather than LacI which can be derepressed by IPTG, we had another transcription factor that represses the device's promoter. Instead, we designed our promoter with flanking sites to be induced by IPTG and unit test it. The idea was to construct everything and plug them all together after we test each component.
Source
Synthesised.