Difference between revisions of "Part:BBa K174011"
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Expressing Spo0A itself alone in the cell would not initiate the sporulation. Spo0A~ P initiates sporulation, first by activating low-threshold genes, and then by gradually increasing and activating high-threshold genes. Hence the multicomponent phosphorelay’s elements, KinA, Spo0F and spo0B, gradually increases spo0A and its activity, and initiates the sporulation. (2). | Expressing Spo0A itself alone in the cell would not initiate the sporulation. Spo0A~ P initiates sporulation, first by activating low-threshold genes, and then by gradually increasing and activating high-threshold genes. Hence the multicomponent phosphorelay’s elements, KinA, Spo0F and spo0B, gradually increases spo0A and its activity, and initiates the sporulation. (2). | ||
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| + | By adding pspac promoter and RBS to the front of KinA, we made our device to express KinA when induced by IPTG. Hence this device will trigger sporulation when IPTG is added to the medium. | ||
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Revision as of 18:21, 13 October 2009
IPTG inducable kinA Bacillus sporulation trigger
Spo0A is the master regulator of sporulation in B. subtilis. When it is phosphorylated, it activates the expression of more than 100 genes(1). It has been shown that, activation of spo0A is sufficient to trigger sporulation.
By artifically synthesising KinA during growth, spoulation can be triggered in a multicomponent phosphorelay in which KinA phosphorylates Spo0F, Spo0F~P in turn phosphorylates Spo0B, and finally Spo0B~P phosphorylates Spo0A. (2)
Expressing Spo0A itself alone in the cell would not initiate the sporulation. Spo0A~ P initiates sporulation, first by activating low-threshold genes, and then by gradually increasing and activating high-threshold genes. Hence the multicomponent phosphorelay’s elements, KinA, Spo0F and spo0B, gradually increases spo0A and its activity, and initiates the sporulation. (2).
By adding pspac promoter and RBS to the front of KinA, we made our device to express KinA when induced by IPTG. Hence this device will trigger sporulation when IPTG is added to the medium.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- Veening, J.-W., W. K. Smits, et al. (2008). "Bistability, Epigenetics, and Bet-Hedging in Bacteria." Annual Review of Microbiology 62(1): 193-210.
- Fujita, M. and R. Losick (2005). "Evidence that entry into sporulation in Bacillus subtilis is governed by a gradual increase in the level and activity of the master regulator Spo0A." Genes & Development 19(18): 2236-2244.