Difference between revisions of "Part:BBa K4169010"
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<center><img src="https://static.igem.wiki/teams/4169/wiki///rna-t/a-rna-thermometer-mutation-2-2.png | <center><img src="https://static.igem.wiki/teams/4169/wiki///rna-t/a-rna-thermometer-mutation-2-2.png | ||
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<center><b>Figure 1. </b>SDS-PAGE analysis of ScGS with His-tag expression </center> | <center><b>Figure 1. </b>SDS-PAGE analysis of ScGS with His-tag expression </center> | ||
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Revision as of 10:13, 12 October 2022
A RNA thermometer mutation 1
Based on the characteristics of secondary structure changes of RNA under different temperature conditions, the RNA thermometer element was introduced in this project. An RC sequence is present on this RNA thermometer, which recruits RNase E to degrade subsequent RNA fragments. At low temperatures,such as 27℃, RNA thermometers will maintain the secondary stem-loop structure, and RC sequences will complement and pair with other bases, unable to recruit RNase, so subsequent sequences will be expressed normally. However, at 37℃, the secondary structure opens, and the subsequent RNA fragments are cleaved and degraded by RNA enzymes.We want to change the temperature at which it functions by mutating its gene sequence.So we mutated its gene sequence, that is, the 8th A in front of ARC was changed to G.
Usage and Biology
iisa
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 39