Difference between revisions of "Part:BBa K4488005"
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<partinfo>BBa_K4488005 short</partinfo>
 
<partinfo>BBa_K4488005 short</partinfo>
  
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Nanobody binding sfGFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. PDB: 3OGO-H. Esp3I golden gate sites flank either end of this part. Analogous to BBa_K929102
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Nanobody binding GFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. PDB: 3OGO-H. Esp3I golden gate sites flank either end of this part. Analogous to BBa_K929102
  
 
<b>Usage:</b>
 
<b>Usage:</b>

Revision as of 10:03, 12 October 2022


Anti-GFP Nanobody/sdAb

Nanobody binding GFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. PDB: 3OGO-H. Esp3I golden gate sites flank either end of this part. Analogous to BBa_K929102

Usage: This part has been used in a composite part with Neae-Intimin for expression in TOP10 E.coli. See part BBa_K4488027.

Modelling of binding of Nanobody H (3OGO-H) to sfGFP made using python package LightDock

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]