Difference between revisions of "Part:BBa K4201019"

Revision as of 10:24, 12 October 2022

crtE-cytoTDS-MBP_T5αOH_RUBY

This composite part is made using a Kan BsaI backbone. The B/W selection was excised during Golden Gate synthesis and replaced with a sequence containing CrtE-cytoTDS-MBP, T5aOH, and RUBY genes. Each inserted gene is flanked by a Gmubi promoter and AtHSP terminator. This construct was made to be transfected into agrobacterium and then into Glycine max. The Crte-cytoTDS-MBP is a fusion protein that codes for the first two enzymes in the paclitaxel biosynthesis pathway. The T5aOH encodes for the third step in the biosynthesis of paclitaxel. The RUBY reporter was added to visualize the construct when integrated into the G. max genome.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 6826
Illegal PstI site found at 994
Illegal PstI site found at 4792
Illegal PstI site found at 5632
Illegal PstI site found at 8365
Illegal PstI site found at 13549
12
INCOMPATIBLE WITH RFC[12]
Unknown
21
INCOMPATIBLE WITH RFC[21]
Unknown
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 6826
Illegal PstI site found at 994
Illegal PstI site found at 4792
Illegal PstI site found at 5632
Illegal PstI site found at 8365
Illegal PstI site found at 13549
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 6826
Illegal PstI site found at 994
Illegal PstI site found at 4792
Illegal PstI site found at 5632
Illegal PstI site found at 8365
Illegal PstI site found at 13549
Illegal NgoMIV site found at 6975
Illegal NgoMIV site found at 9705
Illegal NgoMIV site found at 10284
Illegal NgoMIV site found at 11997
Illegal AgeI site found at 937
Illegal AgeI site found at 2313
Illegal AgeI site found at 5429
Illegal AgeI site found at 5701
Illegal AgeI site found at 6640
Illegal AgeI site found at 8162
Illegal AgeI site found at 8434
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI site found at 940
Illegal BsaI site found at 5432
Illegal BsaI site found at 5704
Illegal BsaI site found at 6643
Illegal BsaI site found at 8165
Illegal BsaI site found at 8437
Illegal BsaI.rc site found at 934
Illegal BsaI.rc site found at 5426
Illegal BsaI.rc site found at 5698
Illegal BsaI.rc site found at 6637
Illegal BsaI.rc site found at 8159
Illegal BsaI.rc site found at 8431
Illegal BsaI.rc site found at 9370
Illegal SapI.rc site found at 3369
Illegal SapI.rc site found at 3575

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4201019 short</partinfo>
-This composite part is made using a Kan BsaI backbone. The B/W selection was excised during Golden Gate synthesis and replaced with a sequence containing CrtE-cytoTDS-MBP, T5aOH, and RUBY genes. Each inserted gene is flanked by a Gmubi promoter and AtHSP terminator. This construct was made to be transfected into agrobacterium and then into Glycine max.
+This composite part is made using a Kan BsaI backbone. The B/W selection was excised during Golden Gate synthesis and replaced with a sequence containing CrtE-cytoTDS-MBP, T5aOH, and RUBY genes. Each inserted gene is flanked by a Gmubi promoter and AtHSP terminator. This construct was made to be transfected into agrobacterium and then into <i>Glycine max</i>.
-The Crte-cytoTDS-MBP is a fusion protein that codes for the first two enzymes in the paclitaxel biosynthesis pathway. The T5aOH encodes for the third step in the biosynthesis of paclitaxel. The RUBY reporter was added to visualize the construct when integrated into the G. max genome.
+The Crte-cytoTDS-MBP is a fusion protein that codes for the first two enzymes in the paclitaxel biosynthesis pathway. The T5aOH encodes for the third step in the biosynthesis of paclitaxel. The RUBY reporter was added to visualize the construct when integrated into the <i>G. max</i> genome.
 <!-- Add more about the biology of this part here