Difference between revisions of "Part:BBa K4477011:Design"

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===Design Notes===
 
===Design Notes===
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Amino acid sequences reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle).
+
Amino acid sequences reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle). Illegal restriction enzymes were removed via codon optimization.
−
 
+
  
 +
For detailed annotations, see Team Virginia 2022's Benchling folder here: https://benchling.com/mrkhoury/f_/YR4zZSUb-virginia-igem-2022-dna-constructs/
  
 
===Source===
 
===Source===

Revision as of 09:47, 12 October 2022


Human/mouse anti-apoB(MDA) (anti-oxLDL) IgG - complete expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 826
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1911
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 455


Design Notes

Amino acid sequences reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle). Illegal restriction enzymes were removed via codon optimization.

For detailed annotations, see Team Virginia 2022's Benchling folder here: https://benchling.com/mrkhoury/f_/YR4zZSUb-virginia-igem-2022-dna-constructs/

Source

Orticumab light chain amino acid sequence acquired from IMGT: https://www.imgt.org/3Dstructure-DB/cgi/details.cgi?pdbcode=9635 Orticumab heavy chain amino acid sequence acquired from IMGT: https://www.imgt.org/3Dstructure-DB/cgi/details.cgi?pdbcode=9635 Mouse heavy chain amino acid sequence acquired from NCBI: Gene ID: 16017


References

1. 10.1038/ncomms9072