Difference between revisions of "Part:BBa K4201020:Design"

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===Design Notes===
 
===Design Notes===
 
Part information and design consideration can be found on respective basic parts pages.
 
Part information and design consideration can be found on respective basic parts pages.
 +
 +
In contrast to other composite parts designed by our group, each individual gene in this assembly has its own promoter and terminator, which allows us to study and compare which combinations of adjoining or separated genes results in the best expression of our target genes.
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Gmubi is constitutive promoter native to <i>Glyine max</i><sup>5</sup>, while AtHSP is the terminator of a heat shock protein that has shown to promote expression in plants<sup>6</sup>.
  
 
===Source===
 
===Source===

Revision as of 09:47, 12 October 2022


CrtE_cytoTDS2__T5αOH_RUBY


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6848
    Illegal PstI site found at 2087
    Illegal PstI site found at 5638
    Illegal PstI site found at 8395
    Illegal PstI site found at 13603
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6848
    Illegal PstI site found at 2087
    Illegal PstI site found at 5638
    Illegal PstI site found at 8395
    Illegal PstI site found at 13603
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 948
    Illegal BsaI site found at 1887
    Illegal BsaI site found at 2167
    Illegal BsaI site found at 3113
    Illegal BsaI site found at 5438
    Illegal BsaI site found at 5718
    Illegal BsaI.rc site found at 934
    Illegal BsaI.rc site found at 1873
    Illegal BsaI.rc site found at 2153
    Illegal BsaI.rc site found at 3100
    Illegal BsaI.rc site found at 5424
    Illegal BsaI.rc site found at 5704
    Illegal BsaI.rc site found at 6651


Design Notes

Part information and design consideration can be found on respective basic parts pages.

In contrast to other composite parts designed by our group, each individual gene in this assembly has its own promoter and terminator, which allows us to study and compare which combinations of adjoining or separated genes results in the best expression of our target genes.

Gmubi is constitutive promoter native to Glyine max5, while AtHSP is the terminator of a heat shock protein that has shown to promote expression in plants6.

Source

CrtE is a GGPP synthase from Pantoea ananatis LMG 20103

RUBY is a reporter gene from the order Caryophyllales.

Gmubi promoters originate from Glycine max.

AtHSP terminators originate from the Arabidopsis thaliana genome.

cytoTDS2 is a taxadiene synthase native to Taxus chinensis var. mairei optimized for use in Glycine max.

T5αOH is a hydroxylase from Taxus baccata that converts taxadiene into taxadiene-5α-ol, a step in the paclitaxel pathway.


de novo Synthesis was completed by iGem sponsors IDT and Twist Biosciences.