Difference between revisions of "Part:BBa K283024"

 
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<partinfo>BBa_K283024 short</partinfo>
 
<partinfo>BBa_K283024 short</partinfo>
  
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ssrB protein under pTet promoter. The ssrB protein can activate the prosseA promoter. The pTet promoter can be repressed by TetR repressor protein. The ssrB coding region does not contain any terminator
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SsrB is autoregulated and activates expression of SPI-2 and SPI-2 co-regulated genes in response to an unknown signal(s). The preliminary data in our lab shows that the activation this regulator depends on the presence of SsrB protein without SsrA on the chromosome of E.coli and salmonella (single copy). However, when we use this prosseA based on the plasmid (not single copy) it can trigger high gene expression level
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:52, 20 October 2009

pTet-rbs-SsrB

SsrB is autoregulated and activates expression of SPI-2 and SPI-2 co-regulated genes in response to an unknown signal(s). The preliminary data in our lab shows that the activation this regulator depends on the presence of SsrB protein without SsrA on the chromosome of E.coli and salmonella (single copy). However, when we use this prosseA based on the plasmid (not single copy) it can trigger high gene expression level


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 88
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]