Difference between revisions of "Part:BBa K4193027:Design"
 
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Figure. Lane1:AtCRE1 gene; Lane2:PTP2+eGFP
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Figure. Lane1:AtCRE1, Lane2:PTP2+eGFP
 
===Source===
 
===Source===
  

Latest revision as of 08:55, 12 October 2022


quorum sensing system (two parts)


Assembly Compatibility:
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    COMPATIBLE WITH RFC[1000]


Design Notes

Cytokinin isopentenyladenine (IP), as quorum sensing molecule, is the product of ATP catalyzed by AtlPT4. When the signal molecule IP reaches a certain concentration, it activates the SSRE promoter by triggering phosphorylation of a series of proteins such as AtCRE1 and Ypd1, and increases the expression of production gene controlled by SSRE promoter. To avoid non-specific HOG pathway induction through Ssk1, we expressed PTP2, the gene encoding an endogenous Hog1 protein phosphatase. In our experiment, to better characterize SSRE promoter and quorum sensing circuit, we put eGFP gene at the downstream of SSRE promoter.

Figure. Lane1:AtCRE1, Lane2:PTP2+eGFP

Source

synthesize from company

References

Yang, X., Liu, J., Zhang, J., Shen, Y., Qi, Q., Bao, X. and Hou, J. (2021). Quorum sensing-mediated protein degradation for dynamic metabolic pathway control in Saccharomyces cerevisiae. Metabolic Engineering, 64, pp.85–94. doi:10.1016/j.ymben.2021.01.010.