Difference between revisions of "Part:BBa K283047:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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| + | <h3>Group: FAFU-CHINA Author: Ruicheng Dai & Changlong Lu Summary: The inductive effects of IPTG in different concentration and time lengths </h3> | ||
| + | <h3>IPTG inductive effect</h3> | ||
| + | <p style="margin-right:100px" align="justify"> | ||
| + | We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect. | ||
| + | </p> | ||
| + | |||
| + | <br /> | ||
| + | <h3>The inductive effect of different concentration IPTG</h3> | ||
| + | We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours | ||
| + | [[File:FAFU-CHINA PART (1).png]] | ||
| + | |||
| + | <h3>The inductive effect of different time length</h3> | ||
| + | We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration. | ||
| + | [[File:FAFU-CHINA PART (2).png]] | ||
Revision as of 11:05, 14 September 2015
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Group: FAFU-CHINA Author: Ruicheng Dai & Changlong Lu Summary: The inductive effects of IPTG in different concentration and time lengths
IPTG inductive effect
We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.
The inductive effect of different concentration IPTG
We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours
.png)
The inductive effect of different time length
We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration.
.png)