Difference between revisions of "Part:BBa K4438160"
Line 3: Line 3:
 
<partinfo>BBa_K4438160 short</partinfo>
 
<partinfo>BBa_K4438160 short</partinfo>
  
−
FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This part is the expression cassette for <partinfo>BBa_K4438100</partinfo> with <partinfo>BBa_J64997</partinfo> upstream. FASTmiR-222-D1_p, after transcription, binds to miR-222, the transcription product of <partinfo>BBa_K4438500</partinfo>. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence.
+
FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This part is the expression cassette for <partinfo>BBa_K4438100</partinfo> with <partinfo>BBa_J64997</partinfo> upstream. FASTmiR-222-D1_p, after transcription, binds to miR-222, the transcription product of <partinfo>BBa_K4438150</partinfo>. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence.
  
  

Revision as of 07:58, 12 October 2022


FASTmiR-222-D1_p

FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This part is the expression cassette for BBa_K4438100 with BBa_J64997 upstream. FASTmiR-222-D1_p, after transcription, binds to miR-222, the transcription product of BBa_K4438150. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence.


Usage and Biology

This part can be used for the detection of miR-222, which proves to be a biomarker for many diseases. The promoter sequence, in the beginning, enhances its usage in both in-vivo and in-vitro conditions. The ON and OFF structure for the sensor was generated as given:

T--IISER-Tirupati India--miRNA 222 1.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 70
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 70
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 70
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 70
  • 1000
    COMPATIBLE WITH RFC[1000]


Notes

The split position of the complementary sequence in the sensor is ACCCAGTAGCCA….GAGTGAGCT

References

Huang, K., Doyle, F., Wurz, Z. E., Tenenbaum, S. A., Hammond, R. K., Caplan, J. L., & Meyers, B. C. (2017). FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs. Nucleic acids research, 45(14), e130-e130