Difference between revisions of "Part:BBa K4477001:Design"

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===Design Notes===
 
===Design Notes===
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Amino acid sequence was reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle).
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Amino acid sequence acquired from database was reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle). Illegal restriction sites were removed via codon optimization.
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+
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===Source===
 
===Source===

Revision as of 07:24, 12 October 2022


Human/mouse anti-apoB(MDA) (anti-oxLDL) IgG antibody - light chain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 382


Design Notes

Amino acid sequence acquired from database was reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle). Illegal restriction sites were removed via codon optimization.

Source

Anti-apoB(MDA) light chain amino acid sequence acquired from IMGT: https://www.imgt.org/3Dstructure-DB/cgi/details.cgi?pdbcode=9635

References