Difference between revisions of "Part:BBa K4229062"

Revision as of 05:52, 12 October 2022

mVenus2 with N-terminal spyCatcher

mVenus2 is with mTurqouise2(BBa_K4229064) one of our reporter genes, with which we tested the building and catching of the minimal(BBa_K4229047) and full wiffleball(BBa_K4229049) and SPD5 (BBa_K4229078). It has the spyCatcher fused on the N-terminal of the protein to make it recruitable inside those compartments.

Principle of catching proteins via Spy/Snp-Catcher by T1 and their incorporation into the BMCs, illustrated as an example of incorporating mVenus in the full wiffleball

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 982
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 415
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4229062 short</partinfo>
-mVenus2 is with mTurqouise2(BBa_K4229064) one of our reporter genes, with which we tested the building and catching of the minimal(BBa_K4229047) and full wiffleball(BBa_K4229049). It has the
+mVenus2 is with mTurqouise2(BBa_K4229064) one of our reporter genes, with which we tested the building and catching of the minimal(BBa_K4229047) and full wiffleball(BBa_K4229049) and SPD5 (BBa_K4229078). It has the spyCatcher fused on the N-terminal of the protein to make it recruitable inside those compartments.
+[[File:ReporterCatched.jpg|800px|thumb|left| Principle of catching proteins via Spy/Snp-Catcher by T1 and their incorporation into the BMCs, illustrated as an example of incorporating mVenus in the full wiffleball]]
 <!-- Add more about the biology of this part here