Difference between revisions of "Part:BBa K4165077"

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===Dry Lab Characterization===
 
===Dry Lab Characterization===
 
This inhibitor was modeled by several software and the top model was acquired by Alphafold2
 
This inhibitor was modeled by several software and the top model was acquired by Alphafold2
−
 
−
===Functional Parameters===
 
−
Isoelectric point (PI): 8.121
 
−
Charge at pH 7: 4.499
 
−
Molecular Weight (Protein): 8.943
 
  
 
Structure assessment results: <br>
 
Structure assessment results: <br>
Line 38: Line 33:
  
 
                   Figure 1.: A graphical illustration showing the structure of the inhibitor (AlphaFold).
 
                   Figure 1.: A graphical illustration showing the structure of the inhibitor (AlphaFold).
 +
 +
===Functional Parameters===
 +
Isoelectric point (PI): 8.121
 +
Charge at pH 7: 4.499
 +
Molecular Weight (Protein): 8.943
  
 
===References===
 
===References===

Revision as of 05:19, 12 October 2022


WAP-four disulphide core domain 10A serine protease inhibitor.

This basic part encodes Human serine protease inhibitor WAP-four disulfide core domain 10A which is able to inhibit HtrA1 (BBa_K4165004).


Usage and Biology

This gene encodes for a type of inhibitor that contains a motif which consists of 8 cysteine residues capable of forming four disulfide bonds at the core of the protease, thus inhibiting its action. This type of inhibitor is very effective and has high affinity for trypsin-like proteases (serine proteases), and in our case it would act as an inhibitor for the trypsin-like catalytic domain of serine protease HtrA1[1]-[3].

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 49
    Illegal PstI site found at 198
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 49
    Illegal PstI site found at 198
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 49
    Illegal PstI site found at 198
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 49
    Illegal PstI site found at 198
  • 1000
    COMPATIBLE WITH RFC[1000]


Dry Lab Characterization

This inhibitor was modeled by several software and the top model was acquired by Alphafold2

Structure assessment results:
Molprobity = 1.62
Q_Mean = 0.6 土 0.1
Ramachandran Favoured = 89.61%
Ramachandran Outliers = 0
Clash Score = 0
C-beta Deviation = 0
Total Score = 5 


                 Figure 1.: A graphical illustration showing the structure of the inhibitor (AlphaFold).

Functional Parameters

Isoelectric point (PI): 8.121 Charge at pH 7: 4.499 Molecular Weight (Protein): 8.943

References

1. Clauss, A., Lilja, H., & Lundwall, Å. (2005). The evolution of a genetic locus encoding small serine proteinase inhibitors. Biochemical and biophysical research communications, 333(2), 383-389. 2. Eigenbrot, C., Ultsch, M., Lipari, M. T., Moran, P., Lin, S. J., Ganesan, R., ... & Kirchhofer, D. (2012). Structural and functional analysis of HtrA1 and its subdomains. Structure, 20(6), 1040-1050. 3. Grau, S., Baldi, A., Bussani, R., Tian, X., Stefanescu, R., Przybylski, M., ... & Ehrmann, M. (2005). Implications of the serine protease HtrA1 in amyloid precursor protein processing. Proceedings of the National Academy of Sciences, 102(17), 6021-6026.