Difference between revisions of "Part:BBa K4441003"
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| − | This is the sequence for the Backward Inner Primer. The BIP consists of a B2 region at the 3'end and a B1c region at the 5'end [2]. The B2 region is complementary to the B2c region of the template sequence. The B1c region is identical to the B1c region of the template sequence [w]. This BIP primer is part of the primer set that detects the WT BRAF: TTACTTACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAATATATTTCTTCATGAAGACCTCACAGTAAAAATAGGTGATTTTGGTCTAGCTACAGTGAAATCTCGATGGA | + | This is the sequence for the Backward Inner Primer (BIP). The BIP consists of a B2 region at the 3'end and a B1c region at the 5'end [2]. The B2 region is complementary to the B2c region of the template sequence. The B1c region is identical to the B1c region of the template sequence [w]. This BIP primer is part of the primer set that detects the WT BRAF: TTACTTACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAATATATTTCTTCATGAAGACCTCACAGTAAAAATAGGTGATTTTGGTCTAGCTACAGTGAAATCTCGATGGA |
GTGGGTCCCATCAGTTTGAACAGTTGTCTGGATCCATTTTGTGGATGGCACCAGAAGTCATCAGAATGCAAGATAAAAATCCATACAGCTTTCAGTCAGATGTATATGCATTTGGAATT | GTGGGTCCCATCAGTTTGAACAGTTGTCTGGATCCATTTTGTGGATGGCACCAGAAGTCATCAGAATGCAAGATAAAAATCCATACAGCTTTCAGTCAGATGTATATGCATTTGGAATT | ||
GTTCTGTATGAATTGATGACTGGACAGTTACCTTATTCAAACATCAACAACAGGGACCAG | GTTCTGTATGAATTGATGACTGGACAGTTACCTTATTCAAACATCAACAACAGGGACCAG | ||
Revision as of 05:34, 12 October 2022
BRAF WT BIP
Sequence used in wetlab experiments: AACAGTTGTCTGGATCCATTTTGTGACATCTGACTGAAAGCTGTA is taken from [1]
Usage and Biology
This is the sequence for the Backward Inner Primer (BIP). The BIP consists of a B2 region at the 3'end and a B1c region at the 5'end [2]. The B2 region is complementary to the B2c region of the template sequence. The B1c region is identical to the B1c region of the template sequence [w]. This BIP primer is part of the primer set that detects the WT BRAF: TTACTTACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAATATATTTCTTCATGAAGACCTCACAGTAAAAATAGGTGATTTTGGTCTAGCTACAGTGAAATCTCGATGGA GTGGGTCCCATCAGTTTGAACAGTTGTCTGGATCCATTTTGTGGATGGCACCAGAAGTCATCAGAATGCAAGATAAAAATCCATACAGCTTTCAGTCAGATGTATATGCATTTGGAATT GTTCTGTATGAATTGATGACTGGACAGTTACCTTATTCAAACATCAACAACAGGGACCAG
Dilutions
Storage Primer Concentration: 100 μM
10X Concentration (Stock): 16 μM
Volume of 10X primer mix: 10 μL
1X Concentration (Final):1.6 μM
Volume of storage primer needed: 1.6 μL
Mixed with F3, B3, FIP for a total of 3.6 μL -- Final primer mix 1 μL of final primer mix is used in the LAMP reaction mix. For more information, visit https://2022.igem.org/Team:Washington
Citations
1. Du, Y., Pothukuchy, A., Gollihar, J. D., Nourani, A., Li, B., & Ellington, A. D. (2017). Coupling sensitive nucleic acid amplification with commercial pregnancy test strips. Angewandte Chemie International Edition, 56(4), 992-996.
2. Loop mediated isothermal amplification - technote. (n.d.). Retrieved October 11, 2022, from http://www.premierbiosoft.com/tech_notes/Loop-Mediated-Isothermal-Amplification.html Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]