Difference between revisions of "Part:BBa K4274008"
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Guide RNA (gRNA) is used as a molecule that helps with the cleavage of DNA since it guides the Cas nuclease to a targeted dsDNA sequence. As a gRNA, sfp_target is used for knocking-out the natural, invalid sfp gene, and then knocking-in sfp (Part number:BBa_K4274009) and degQ(Part number:BBa_K4274010) gene in the genome of <i>Bacillus subtilis</i>. | Guide RNA (gRNA) is used as a molecule that helps with the cleavage of DNA since it guides the Cas nuclease to a targeted dsDNA sequence. As a gRNA, sfp_target is used for knocking-out the natural, invalid sfp gene, and then knocking-in sfp (Part number:BBa_K4274009) and degQ(Part number:BBa_K4274010) gene in the genome of <i>Bacillus subtilis</i>. | ||
Revision as of 04:54, 12 October 2022
sfp_target(gRNA)
Guide RNA (gRNA) is used as a molecule that helps with the cleavage of DNA since it guides the Cas nuclease to a targeted dsDNA sequence. As a gRNA, sfp_target is used for knocking-out the natural, invalid sfp gene, and then knocking-in sfp (Part number:BBa_K4274009) and degQ(Part number:BBa_K4274010) gene in the genome of Bacillus subtilis.
Source
Bacillus subtilis
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]