Difference between revisions of "Part:BBa K4509569"
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<partinfo>BBa_K4509569 short</partinfo> | <partinfo>BBa_K4509569 short</partinfo> | ||
− | The enzyme horseradish peroxidase, found in the roots of horseradish catalyzes the oxidation of various organic substrates by hydrogen peroxide. It is a metalloenzyme with many isoforms; the most studied type is C. The composite part starts with the | + | The enzyme horseradish peroxidase, found in the roots of horseradish catalyzes the oxidation of various organic substrates by hydrogen peroxide. It is a metalloenzyme with many isoforms; the most studied type is C. The composite part starts with the [https://parts.igem.org/Part:BBa_J23100 BBa_J23100] constitutive promoter, continues with RBS [https://parts.igem.org/Part:BBa_B0034 BBa_B0034] and is followed by the Horseradish Peroxidase coding sequence [https://parts.igem.org/Part:BBa_K1291571 BBa_K1291571]. The sequence ends with a terminator and a BamH1 restriction site. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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=Characterization= | =Characterization= | ||
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==Cell Burden== | ==Cell Burden== | ||
J23116 has a burden range of -0.6 ± 11.0% | J23116 has a burden range of -0.6 ± 11.0% | ||
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+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K4509569 SequenceAndFeatures</partinfo> | ||
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+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K4509569 parameters</partinfo> | ||
+ | <!-- --> |
Revision as of 07:59, 12 October 2022
HORSERADISH PEROXIDASE with constitutive promoter J23100
The enzyme horseradish peroxidase, found in the roots of horseradish catalyzes the oxidation of various organic substrates by hydrogen peroxide. It is a metalloenzyme with many isoforms; the most studied type is C. The composite part starts with the BBa_J23100 constitutive promoter, continues with RBS BBa_B0034 and is followed by the Horseradish Peroxidase coding sequence BBa_K1291571. The sequence ends with a terminator and a BamH1 restriction site.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 445
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 565
Illegal AgeI site found at 719
Illegal AgeI site found at 1022 - 1000COMPATIBLE WITH RFC[1000]