Difference between revisions of "Part:BBa K4444010"
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<partinfo>BBa_K4444010 short</partinfo>
 
<partinfo>BBa_K4444010 short</partinfo>
  
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PhoCl Shed mRuby is  modified from the RELEASE system. It contains a mRuby and a tri-transmembrane domain; the ER retention motif, furin cut site, GFP, and HCV cut site were all removed.
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PhoCl Shed mRuby is  modified from the RELEASE system. It contains a mRuby as a protein of interest, PhoCl2c, and a tri-transmembrane domain; the ER retention motif, furin cut site, GFP, and HCV cut site were all removed. PhoCl Shed will keep the protein of interest on the cell membrane until violet light is shined on it, which then breaks PhoCl and releases all the protein from the surface of the cell membrane.  
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 03:52, 12 October 2022


PhoCl Shed mRuby

PhoCl Shed mRuby is modified from the RELEASE system. It contains a mRuby as a protein of interest, PhoCl2c, and a tri-transmembrane domain; the ER retention motif, furin cut site, GFP, and HCV cut site were all removed. PhoCl Shed will keep the protein of interest on the cell membrane until violet light is shined on it, which then breaks PhoCl and releases all the protein from the surface of the cell membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 769
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1654
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1288
    Illegal SapI.rc site found at 73