Difference between revisions of "Part:BBa K4156113"

Latest revision as of 02:51, 12 October 2022

plac-mRFP

plac-mRFP is a biobrick that expresses mRFP controlled by the plac operon ( BBa_R0010 ). plac-mRFP functions as a red fluorescent signal to characterize the performance of the plac operon

Usage and Biology

In this biobrick, we used the plac operon to regulate downstream mRFP expression and eventually introduced the rrnB T1 and T7Te dual terminators. this design allowed the spatiotemporal intensity of plac manipulator-regulated gene expression to be measured by the red fluorescent signal. We were thus able to verify the specific response of the plac operon to lactose.

Characterization

We first successfully isolated this part and verified it by gel electrophoresis before fusing mRFP with plac.

Enhancement of E. coli EcN tropism for colorectal cancer

To make the engineered strain nengg better adhere to colorectal cancer cells, we used engineered bacteria carrying the adhesion proteins INP-HlpA and mRFP, and the fluorescence was significantly enhanced compared to the control engineered bacteria expressing mRFP alone, indicating that the HlpA protein could effectively improve the adhesion of EcN to colorectal cells. (where CT26 and RKO were magnified 400-fold, while RKO was magnified 300-fold), and this change was particularly evident in RKO cells.

Sequence and Features