Difference between revisions of "Part:BBa K4438601"
Line 7: Line 7:
  
 
===Usage and Biology===
 
===Usage and Biology===
−
Few bases of this part are complementary (Watson-Crick base pairing) to T6 (BBa_K4438600) and are  designed to block its sequence motif. Motif, mostly associated with the loops, is linked to the formation of the specific binding site for testosterone [1]. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. The hormone binds with T6 (BBa_K4438600) with high affinity and displaces the T6_Trigger_1_phi29.
+
Few bases of this part are complementary (Watson-Crick base pairing) to T6 <partinfo>BBa_K4438600</partinfo> and are  designed to block its sequence motif. Motif, mostly associated with the loops, is linked to the formation of the specific binding site for testosterone [1]. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. The hormone binds with T6 <partinfo>BBa_K4438600</partinfo> with high affinity and displaces the T6_Trigger_1_phi29.
−
This part has complete complementarity with the part T6_Binder_1(BBa_K4438602). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers.
+
This part has complete complementarity with the part T6_Binder_1(<partinfo>BBa_K4438602</partinfo>). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers.
 
Different levels of testosterone, an anabolic androgenic steroid (AAS), in biological fluids can be detected using all these three parts.
 
Different levels of testosterone, an anabolic androgenic steroid (AAS), in biological fluids can be detected using all these three parts.
−
 
+
[[File:T--IISER-Tirupati_India--T6_1.png]]
  
 
<!-- -->
 
<!-- -->

Revision as of 09:23, 12 October 2022

T6_Trigger_1_midtrigger_phi29


T6_Trigger_1_phi29 is single-stranded DNA having 27 nucleotides. Figure 1B) Illustrates the secondary structure and its minimum free energy.


Usage and Biology

Few bases of this part are complementary (Watson-Crick base pairing) to T6 BBa_K4438600 and are designed to block its sequence motif. Motif, mostly associated with the loops, is linked to the formation of the specific binding site for testosterone [1]. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. The hormone binds with T6 BBa_K4438600 with high affinity and displaces the T6_Trigger_1_phi29. This part has complete complementarity with the part T6_Binder_1(BBa_K4438602). Phi 29 DNA extension polymerase extends the template strand and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. Different levels of testosterone, an anabolic androgenic steroid (AAS), in biological fluids can be detected using all these three parts. T--IISER-Tirupati India--T6 1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Skouridou, V., Jauset-Rubio, M., Ballester, P., Bashammakh, A. S., El-Shahawi, M. S., Alyoubi, A. O., & O’Sullivan, C. K. (2017). Selection and characterization of DNA aptamers against the steroid testosterone. Microchimica Acta, 184(6), 1631-1639.