Difference between revisions of "Part:BBa K4060021"
(Undo revision 637410 by XueZhang (talk))
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<partinfo>BBa_K4060021 short</partinfo>
 
<partinfo>BBa_K4060021 short</partinfo>
  
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pelB leader sequence with aprN used signal peptide gene of PelB Helps nattokinase secretion. The propeptide has been proved to function as an intramolecular chaperone and is essential for guiding the correct folding of the linked protein to its mature form.  
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The expression of Nattokinase in Escherichia coli is prone to the formation of inclusion bodies, so we use weak promoter and RBS combination to express Nattokinase. In order to have enough time and space to fold and secrete nattokinase into the intestine.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:33, 12 October 2022


pelB leader sequence with aprN (without native signal peptide sequence)

The expression of Nattokinase in Escherichia coli is prone to the formation of inclusion bodies, so we use weak promoter and RBS combination to express Nattokinase. In order to have enough time and space to fold and secrete nattokinase into the intestine.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 67
    Illegal NotI site found at 211
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 67
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 345
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 349