Difference between revisions of "Part:BBa K4204002"

Revision as of 00:46, 12 October 2022

Description

This sequence contains 3 protection bases at the 5'end of the sequence, which is to protect the sequence from damage. And immediately following the T7 promoter, connecting with the sgRNA scaffold, which helps the folding of sgRNA. And at the 3'end, the target-16s region pairs with the targeted B.cereus 16srRNA sequence and enables cleavage.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 26
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 26
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 26
1000
COMPATIBLE WITH RFC[1000]

Revision as of 00:45, 12 October 2022 (view source) Jianfei Song (Talk \| contribs) ← Older edit		Revision as of 00:46, 12 October 2022 (view source) Jianfei Song (Talk \| contribs) (→‎Description) Newer edit →
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	==Description==		==Description==

−	This sequence contains 3 protection bases at the 5’ end of the sequence, which is to protect the sequence from damage. And immediately following the T7 ~~promoter，~~ connecting with the sgRNA scaffold, which helps the folding of sgRNA. And at the 3’ end, the target-16s region pairs with the targeted B.cereus 16srRNA sequence and enables cleavage.	+	This sequence contains 3 protection bases at the 5'end of the sequence, which is to protect the sequence from damage. And immediately following the T7 promoter, connecting with the sgRNA scaffold, which helps the folding of sgRNA. And at the 3'end, the target-16s region pairs with the targeted B.cereus 16srRNA sequence and enables cleavage.