Difference between revisions of "Part:BBa K4439007"
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====Protein Purification using Ni-NTA Beads==== | ====Protein Purification using Ni-NTA Beads==== | ||
| − | Materials | + | *Materials* |
. 2.5M imidazole stock solution | . 2.5M imidazole stock solution | ||
. 5M NaCl solution | . 5M NaCl solution | ||
| + | |||
. Protease inhibitor tablet | . Protease inhibitor tablet | ||
| + | |||
. Turbonuclease | . Turbonuclease | ||
| + | |||
. Sonicator | . Sonicator | ||
| + | |||
. 1M HEPES | . 1M HEPES | ||
| + | |||
. HisPur Ni-NTA beads in 20% ethanol | . HisPur Ni-NTA beads in 20% ethanol | ||
| + | |||
. Disposable plastic column | . Disposable plastic column | ||
| + | |||
. Centrifuge going to 20 000 xg | . Centrifuge going to 20 000 xg | ||
| + | |||
. 0.30 µm filters | . 0.30 µm filters | ||
| + | |||
. Tube of bacterial culture induced with IPTG | . Tube of bacterial culture induced with IPTG | ||
Revision as of 22:10, 11 October 2022
mSA-N[AS]4C-CBD-10xHis
Contents
Abstract
To complete
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Protein's Characterization
Description
To complete
Usage and Biology
To complete
Modeling
To complete
Experiments
Adapted Protocols
Protein Purification using Ni-NTA Beads
- Materials*
. 2.5M imidazole stock solution
. 5M NaCl solution
. Protease inhibitor tablet
. Turbonuclease
. Sonicator
. 1M HEPES
. HisPur Ni-NTA beads in 20% ethanol
. Disposable plastic column
. Centrifuge going to 20 000 xg
. 0.30 µm filters
. Tube of bacterial culture induced with IPTG
Lab's Tips and Tricks
To complete
Results
Expression
To complete
Purification
To complete
Biofilm Fabrication
To complete
Hydrophobicity tests
To complete