Difference between revisions of "Part:BBa K4229001"

Revision as of 21:31, 11 October 2022

Fre (NAD(P)H-flavin reductase)

The flavin-reducing enzyme Fre catalyzes the reduction of soluble flavins by reduced pyridine nucleotides (UniProt ID:P0AEN1).

Fre is one of four enzymes we used in our project to produce indigo and indirubin. In our pathway it accelerates the recycling of the cofactor NADPH, and adds more FADH2 for the enzyme XiaI (Figure 1).

Figure 1: Schematic representation of the used Indigo/Indirubin pathway. L-Tryptophan is imported by the membrane protein TnaB (low affinity tryptophan permease). L-tryptophan is cleaved into Indole, NH4+ and Pyruvate by the Tryptophanase TnaA. The reactions continues by the hydroxylation of Indole through XiaI. To enhance the effectivity of this enzyme, the NAD(P)H-flavin reductase provides XiaI with FADH2 by adding Hydrogen to FAD. Finally, Indole is transformed to either 3-Hydroxyindole or 2-Hydroxyindole. These two substances spontaneously react to 3-Oxindole and 2-Oxindole through the secession of hydrogen from the OH-group. Through spontaneous dimerization Indigo and Indirubin are formed. Graphic adapted from [63].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 304
Illegal SapI.rc site found at 424

@@ Line 7: / Line 7: @@
 The flavin-reducing enzyme Fre catalyzes the reduction of soluble flavins by reduced pyridine nucleotides (UniProt ID:P0AEN1). <br>
 <br>
-Fre is one of four enzymes we used in our project to produce indigo and indirubin. In our pathway it accelerates the recycling of the cofactor NADPH, and adds more FADH2 for the enzyme XiaI.
+Fre is one of four enzymes we used in our project to produce indigo and indirubin. In our pathway it accelerates the recycling of the cofactor NADPH, and adds more FADH2 for the enzyme XiaI (Figure 1).
 [[File:Grafik indigo weier bg.png|900px|thumb|left|]]
+<b>Figure 1: Schematic representation of the used Indigo/Indirubin pathway.</b> L-Tryptophan is imported by the membrane protein TnaB (low affinity tryptophan permease). L-tryptophan is cleaved into Indole, NH4+ and Pyruvate by the Tryptophanase TnaA. The reactions continues by the hydroxylation of Indole through XiaI. To enhance the effectivity of this enzyme, the NAD(P)H-flavin reductase provides XiaI with FADH2 by adding Hydrogen to FAD. Finally, Indole is transformed to either 3-Hydroxyindole or 2-Hydroxyindole. These two substances spontaneously react to 3-Oxindole and 2-Oxindole through the secession of hydrogen from the OH-group. Through spontaneous dimerization Indigo and Indirubin are formed. Graphic adapted from [63].
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