Difference between revisions of "Part:BBa K4323000"

Revision as of 19:43, 11 October 2022

Coding sequence for Aerolysin

Secreted, cytolytic toxin that forms pores in host membranes after proteolytic removal of a C-terminal propeptide, leading to destruction of the membrane permeability barrier and host cell death. The pores are formed by transmembrane beta-strands and are approximately 3 nm in diameter. This part has been codon optimized for expression in Escherichia coli.

Figure 1. The toxicity of Aerolysin to Dreissena bugensis.

Figure 2. His-Trap Ni-column purified Aerolysin. L: ladder, C5: 10 % B wash, D1: fraction of gradient at ~30 % B, D4: fraction of gradient at ~50 % B, D8: elution at 100 % B. A buffer: 20 mM Tris-HCl, 200 mM NaCl, pH 8.0; B buffer: 20 mM Tris-HCl, 200 mM NaCl, 300 mM imidazole, pH 8.0. Arrow: purified Aerolysin protein, MW: 55.2 kDa.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 206
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 75
Illegal AgeI site found at 313
Illegal AgeI site found at 1270
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 '''Figure 1. The toxicity of Aerolysin to <i>Dreissena bugensis</i>.'''
-[[File:Ni-purified Aerolysin SDS-PAGE.png|800px|center|Ni-purified Aerolysin SDS-PAGE]]
+[[File:Ni-purified Aerolysin SDS-PAGE.png|400px|center|Ni-purified Aerolysin SDS-PAGE]]
 '''Figure 2. His-Trap Ni-column purified Aerolysin.  L: ladder, C5: 10 % B wash, D1: fraction of gradient at ~30 % B, D4: fraction of gradient at ~50 % B, D8: elution at 100 % B.  A buffer: 20 mM Tris-HCl, 200 mM NaCl, pH 8.0; B buffer: 20 mM Tris-HCl, 200 mM NaCl, 300 mM imidazole, pH 8.0.  Arrow: purified Aerolysin protein, MW: 55.2 kDa.'''