Difference between revisions of "Part:BBa K3876501:Design"
 
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===<b> Lambert iGEM 2022: hsa-miR-122-5p </b>===
 
===<b> Lambert iGEM 2022: hsa-miR-122-5p </b>===
 
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Latest revision as of 19:24, 11 October 2022


mimic miRNA-122

Lambert iGEM 2022: hsa-miR-122-5p


Sequence of hsa-miR-122-5p: UGGAGUGUGACAAUGGUGUUUG

The sequence of hsa-miR-122-5p was taken from miRBase, and we chose the mature sequence as it is found in circulation. No changes were made as these were the target sequence.

References:

Mature sequence hsa-miR-122-5p. Mirbase. (n.d.). Retrieved October 10, 2022, from https://www.mirbase.org/cgi-bin/mirna_entry.pl?acc=MIMAT0000421





Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This mimic RNA will be used as a target to detect in a mimic body fluid.


Source

miRBase: https://www.mirbase.org/cgi-bin/mirna_entry.pl?acc=MI0000442

References