Difference between revisions of "Part:BBa K4117088"
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<partinfo>BBa_K4117088 short</partinfo>
 
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“T7 promoter+ PmrA+ RBS+ PmrB+ rnaB T1 terminator+ pmrC promoter” is the THC sensing pathway. To alert vaccinators that they have ingested THC, we design a THC sensing system. In this pathway, anti-THC can specifically bind to THC, and then promote PmrB phosphorylation of PmrA. PmrC promoters activate phosphorylated PmrA proteins and initiate transcription to express pigment proteins. Pigment proteins can be excreted in the stool, acting as a warning.Outer membrane protease T (OmpT) is a 33.5 kDa endoprotease located on the outer membrane of Escherichia coli. OmpT signal peptide is an excretion tag linked on the N-terminus of protein. It can transport proteins across the outer membrane with the help of OmpT.Δ9-THC is hydroxylated to 11-OH-Δ9-THC via CYP2C9 (BBa_K4117002) and CYP2C19(BBa_K4117022), remaining psychoactive.
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“T7 promoter+ pmrA+ RBS+ pmrB+ rrnB T1 terminator+ pmrC promoter” is the THC sensing pathway. To alert vaccinators that they have ingested THC, we design a THC sensing system. In this pathway, anti-THC can specifically bind to THC, and then promote PmrB phosphorylation of PmrA. PmrC promoters activate phosphorylated PmrA proteins and initiate transcription to express pigment proteins. Pigment proteins can be excreted in the stool, acting as a warning.Outer membrane protease T (OmpT) is a 33.5 kDa endoprotease located on the outer membrane of Escherichia coli. OmpT signal peptide is an excretion tag linked on the N-terminus of protein. It can transport proteins across the outer membrane with the help of OmpT.Δ9-THC is hydroxylated to 11-OH-Δ9-THC via CYP2C9 (BBa_K4117002) and CYP2C19(BBa_K4117022), remaining psychoactive.
  
 
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Revision as of 06:24, 12 October 2022


Status: 500 Content-type: text/html

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“T7 promoter+ pmrA+ RBS+ pmrB+ rrnB T1 terminator+ pmrC promoter” is the THC sensing pathway. To alert vaccinators that they have ingested THC, we design a THC sensing system. In this pathway, anti-THC can specifically bind to THC, and then promote PmrB phosphorylation of PmrA. PmrC promoters activate phosphorylated PmrA proteins and initiate transcription to express pigment proteins. Pigment proteins can be excreted in the stool, acting as a warning.Outer membrane protease T (OmpT) is a 33.5 kDa endoprotease located on the outer membrane of Escherichia coli. OmpT signal peptide is an excretion tag linked on the N-terminus of protein. It can transport proteins across the outer membrane with the help of OmpT.Δ9-THC is hydroxylated to 11-OH-Δ9-THC via CYP2C9 (BBa_K4117002) and CYP2C19(BBa_K4117022), remaining psychoactive.

Sequence and Features Status: 500 Content-type: text/html

Software error:

Can't find string terminator '"' anywhere before EOF at /websites/parts.igem.org/cgi/lib/Range.pm line 311.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

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