Difference between revisions of "Part:BBa K4117888:Design"

(→‎Design Notes)
(→‎Source)
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===Source===
 
===Source===
  
−
how to make UGT1A3
+
We design the DNA sequence based on the amino acid sequence according to E·coli codon preference.The amino acid sequence is from UniProt:P35503
 +
 
 +
C-phycocyanin alpha subunit and beta subunit genes come from Part:BBa_K4117779.
 +
 
 +
PmrC promoter comes from Part:BBa_K1459006.
 +
 
 +
PmrA gene comes from Part:BBa_K3611003.
 +
 
 +
OmpT signal peptide gene comes from Part:BBa_K3805532.
 +
 
 +
OmpA signal peptide gene comes from Part:BBa_K103006.
 +
 
 +
T7 terminator gene comes from Part:BBa_B0012.
 +
 
 +
rrnB T1 Terminator gene comes from BBa_K3257020.
  
 
===References===
 
===References===

Revision as of 17:47, 11 October 2022


UGT1A3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4599
    Illegal XbaI site found at 693
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4599
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4599
    Illegal BglII site found at 4257
    Illegal BamHI site found at 2887
    Illegal BamHI site found at 3444
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4599
    Illegal XbaI site found at 693
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4599
    Illegal XbaI site found at 693
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
    Illegal AgeI site found at 3031
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 732


Design Notes

To degrade Δ9-THC, we added a pathway to sense Δ9-THC in front of the UGT1A3 gene.

Source

We design the DNA sequence based on the amino acid sequence according to E·coli codon preference.The amino acid sequence is from UniProt:P35503

C-phycocyanin alpha subunit and beta subunit genes come from Part:BBa_K4117779.

PmrC promoter comes from Part:BBa_K1459006.

PmrA gene comes from Part:BBa_K3611003.

OmpT signal peptide gene comes from Part:BBa_K3805532.

OmpA signal peptide gene comes from Part:BBa_K103006.

T7 terminator gene comes from Part:BBa_B0012.

rrnB T1 Terminator gene comes from BBa_K3257020.

References