Difference between revisions of "Part:BBa K4165013"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This gene encodes a homolog of ubiquitin-conjugating enzyme E2 variant 1. These ubiquitin-conjugating enzymes don’t have the conserved cysteine residue critical for the catalytic activity of E2s. Based on the specific E2 used, the E2 enzymes can direct the ubiquitination process to different subsets of ubiquitin lysines. In our case, we will use its interaction with the UBE2N (E2 ligase) to catalyze the formation of polyubiquitin chains and the degradation of our targeted proteins. | + | This gene encodes a homolog of ubiquitin-conjugating enzyme E2 variant 1. These ubiquitin-conjugating enzymes don’t have the conserved cysteine residue critical for the catalytic activity of E2s. Based on the specific E2 used, the E2 enzymes can direct the ubiquitination process to different subsets of ubiquitin lysines. The formation of UBE2N/UBE2V2 complex fascilitates the elongation of ubiquitination to forma a ployubiquitin chain. In our case, we will use its interaction with the UBE2N (E2 ligase) to catalyze the formation of polyubiquitin chains and the degradation of our targeted proteins. |
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===<span class='h3bb'>Sequence and Features</span>=== | ===<span class='h3bb'>Sequence and Features</span>=== | ||
<partinfo>BBa_K4165013 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4165013 SequenceAndFeatures</partinfo> | ||
Revision as of 20:30, 11 October 2022
UBE2V2
Ubiquitin-conjugating E2 ligase that has a role in the ubiquitination cascade for protein degradation.
Usage and Biology
This gene encodes a homolog of ubiquitin-conjugating enzyme E2 variant 1. These ubiquitin-conjugating enzymes don’t have the conserved cysteine residue critical for the catalytic activity of E2s. Based on the specific E2 used, the E2 enzymes can direct the ubiquitination process to different subsets of ubiquitin lysines. The formation of UBE2N/UBE2V2 complex fascilitates the elongation of ubiquitination to forma a ployubiquitin chain. In our case, we will use its interaction with the UBE2N (E2 ligase) to catalyze the formation of polyubiquitin chains and the degradation of our targeted proteins.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
WetLab Results
Transformation of His UBE2V2 in BL-21 using pGS-21a vector
Figure 1. Transformed plate of His UBE2v2 + pGS-21a
Transformation of His UBE2V2 in DH-5 alpha
Figure 2. Transformed plate of His UBE2V2 + pJET
Refrences
1. UBE2V2 ubiquitin conjugating enzyme E2 v2 [homo sapiens (human)] - gene - NCBI. (n.d.). Retrieved September, from https://www.ncbi.nlm.nih.gov/gene/7336
2. David, Y., Ziv, T., Admon, A., & Navon, A. (2010). The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines. Journal of Biological Chemistry, 285(12), 8595-8604.
3. Pharos: Ubiquitin-conjugating enzyme E2 N (Tchem). (2022).