Difference between revisions of "Part:BBa K4165031"
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The switch was modeled by (Alphafold - Rosettafold - tRrosetta) and the top model was obtained from tRrosseta with a score of 4 out of 6 according to our quality assessment code. | The switch was modeled by (Alphafold - Rosettafold - tRrosetta) and the top model was obtained from tRrosseta with a score of 4 out of 6 according to our quality assessment code. | ||
+ | <html> | ||
+ | <style> | ||
+ | table, th, td { | ||
+ | border:1px solid black; margin-left:auto;margin-right:auto; | ||
+ | } | ||
+ | </style> | ||
+ | <body> | ||
+ | <table style="width:65%"> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <th>cbeta_deviations</th> | ||
+ | <th>clashscore</th> | ||
+ | <th>molprobity</th> | ||
+ | <th>ramachandran_favored</th> | ||
+ | <th>ramachandran_outliers</th> | ||
+ | <th>Qmean_4</th> | ||
+ | <th>Qmean_6</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>0</td> | ||
+ | <td>3.76</td> | ||
+ | <td>1.5</td> | ||
+ | <td>95.17</td> | ||
+ | <td>0.69</td> | ||
+ | <td>-0.31007</td> | ||
+ | <td>-1.21262</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </body> | ||
+ | </html> | ||
Revision as of 13:55, 13 October 2022
HtrA1 switch 11
This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), 6x His-tag (BBa_K4165020), SPINK8 Inhibitor (BBa_K4165010), TD28rev (BBa_K4165006), WWW (BBa_K4165007), and T7 terminator (BBa_K731721).
Usage and Biology
Switch 11 is used to mediate the activity of HTRA1. It is composed of 3 parts connected by different linkers; an HtrA1 PDZ peptide, a clamp of two targeting peptides for tau or amyloid beta, and a catalytic domain inhibitor. Activating HTRA1 requires a conformational change in the linker, eliminating the attached inhibitor from the active site. The conformational rearrangement can be mediated through the binding of affinity clamp to tau or beta-amyloid. This binding will result in a tension that detaches the inhibitor from the active site.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 528
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Dry lab
Modeling
The switch was modeled by (Alphafold - Rosettafold - tRrosetta) and the top model was obtained from tRrosseta with a score of 4 out of 6 according to our quality assessment code.
cbeta_deviations | clashscore | molprobity | ramachandran_favored | ramachandran_outliers | Qmean_4 | Qmean_6 |
---|---|---|---|---|---|---|
0 | 3.76 | 1.5 | 95.17 | 0.69 | -0.31007 | -1.21262 |
Figure 1. The 3D structure of switch 11 visualized by Pymol.