Difference between revisions of "Part:BBa K4192143:Experience"
Line 5: Line 5:
  
 
===Applications of BBa_K4192143===
 
===Applications of BBa_K4192143===
−
 
+
The construction was unsuccessful, and PcysH was skipped directly in gibson assembly to construct PcysJ-mazE-mazF, probably due to the homologous arm design
−
When tried in Pseudomonas fluorescein, the promoter name showed no inducibility and 85% of the bacteria died directly. Probably because the design is not suitable for bacteria other than E. coli.
+
  
 
===User Reviews===
 
===User Reviews===

Revision as of 16:44, 11 October 2022


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4192143

The construction was unsuccessful, and PcysH was skipped directly in gibson assembly to construct PcysJ-mazE-mazF, probably due to the homologous arm design

User Reviews

UNIQb024339d919e2867-partinfo-00000000-QINU UNIQb024339d919e2867-partinfo-00000001-QINU