Difference between revisions of "Part:BBa K4192143:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The construction was unsuccessful, and PcysH was skipped directly in gibson assembly to construct PcysJ-mazE-mazF, probably due to the homologous arm design | |
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | All individual components were derived from E. coli. | |
| − | + | ||
| − | + | ||
===References=== | ===References=== | ||
[1]. Liu, Y.N., et al., Developing a high-throughput screening method for threonine overproduction based on an artificial promoter. Microbial Cell Factories, 2015. 14(1). | [1]. Liu, Y.N., et al., Developing a high-throughput screening method for threonine overproduction based on an artificial promoter. Microbial Cell Factories, 2015. 14(1). | ||
Latest revision as of 04:45, 12 October 2022
PcysJ-PcysH-mazE-mazF
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 482
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The construction was unsuccessful, and PcysH was skipped directly in gibson assembly to construct PcysJ-mazE-mazF, probably due to the homologous arm design
Source
All individual components were derived from E. coli.
References
[1]. Liu, Y.N., et al., Developing a high-throughput screening method for threonine overproduction based on an artificial promoter. Microbial Cell Factories, 2015. 14(1).