Difference between revisions of "Part:BBa K4365019"
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| − | Alpha mating factor is a highly secreted protein from S. cerevisiae. A signal peptide from such proteins increases the secretion of a protein of interest in yeast if the signal peptide signal is placed before it. | + | Alpha mating factor is a highly secreted protein from S. cerevisiae. A signal peptide from such proteins increases the secretion of a protein of interest in yeast if the signal peptide signal is placed before it. The α-mating factor pre-pro signal peptide has been previously shown to be able to secrete proteins<ref>Peñas M. M. et al. (1998) Identification, characterization, and In situ detection of a fruit-body-specific hydrophobin of Pleurotus ostreatus, Appl Environ Microbiol. 64(10):4028-34. doi: 10.1128/AEM.64.10.4028-4034.1998. PMID: 9758836; PMCID: PMC106595.</ref>. |
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Latest revision as of 22:53, 11 October 2022
Alpha mating factor signal peptide from Saccharomyces cerevisiae
Alpha mating factor is a highly secreted protein from S. cerevisiae. A signal peptide from such proteins increases the secretion of a protein of interest in yeast if the signal peptide signal is placed before it. The α-mating factor pre-pro signal peptide has been previously shown to be able to secrete proteins[1].
| ID | Source | Species | Prediction coefficient [2] | turboRFP secretion | |
|---|---|---|---|---|---|
| sp1 | BBa_K4365006 | MPGI | Magnaporthe grisea | 0.9562 | Yes |
| sp2 | BBa_K4365007 | RodA | Aspergillus nidulans | 0.9553 | Yes |
| sp3 | BBa_K4365008 | HYPI | Aspergillus fumigatus | 0.9703 | No |
| sp4 | BBa_K4365009 | SsgA | Metarhizium anisopliae | 0.9126 | No |
| sp5 | BBa_K4365010 | HCF-4 | Cladosporium fulvum | 0.9126 | Yes |
| sp6 | BBa_K4365025 | HCF-5 | Cladosporium fulvum | 0.9234 | NA |
| sp7 | BBa_K4365026 | CCG-2 | Neurospora crassa | 0.9566 | NA |
| sp8 | BBa_K4365011 | HCF-1 | Cladosporium fulvum | 0.6850 | Yes |
| sp9 | BBa_K4365027 | HCF-2 | Cladosporium fulvum | 0.8144 | NA |
| sp10 | BBa_K4365012 | HCF-3 | Cladosporium fulvum | 0.7934 | No |
| sp11 | BBa_K4365013 | SC3 | Schizophyllum commune | 0.6327 | No |
| sp12 | BBa_K4365014 | ABH3 | Agaricus bisporus | 0.9458 | Yes |
| sp13 | BBa_K4365028 | COH1 | Coprinus cinereus | 0.8545 | NA |
| sp14 | BBa_K4365015 | FBH1 | Pleurotus ostreatus | 0.3367 | Yes |
| sp15 | BBa_K4365016 | Aa-PRI2 | Agrocybe aegerita | 0.6356 | Yes |
| sp16 | BBa_K4365033 | Hyd-Pt1 | Pisolithus tinctorius | 0.8352 | NA |
| sp17 | BBa_K4365017 | HFBI | Trichoderma reesei | 0.9851 | Yes |
| sp18 | BBa_K4365018 | HFBII | Trichoderma reesei | 0.6862 | Yes |
| sp19 | BBa_K4365019 | α-mating factor | Saccharomyces cerevisiae | 0.9988 | Yes |
- ↑ Peñas M. M. et al. (1998) Identification, characterization, and In situ detection of a fruit-body-specific hydrophobin of Pleurotus ostreatus, Appl Environ Microbiol. 64(10):4028-34. doi: 10.1128/AEM.64.10.4028-4034.1998. PMID: 9758836; PMCID: PMC106595.
- ↑ José Juan Almagro Armenteros et al. (2019) SignalP 5.0 improves signal peptide predictions using deep neural networks Nature Biotechnology, 37, 420-423, doi: 10.1038/s41587-019-0036-z
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 23
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 23
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 23
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 23
- 1000COMPATIBLE WITH RFC[1000]