Difference between revisions of "Part:BBa K4509420:Design"
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===References=== | ===References=== |
Revision as of 15:04, 11 October 2022
HORSERADISH PEROXIDASE with constitutive promoter J23116
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 445
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 565
Illegal AgeI site found at 719
Illegal AgeI site found at 1022 - 1000COMPATIBLE WITH RFC[1000]
Source and Improvement
The promoter J23119 is replaced with J23116. This switch in the design will reduce the burden in the cell during overexpression of enzymes
Characterization
To be added File:HRPgraph.jpg