Difference between revisions of "Part:BBa K4169023"

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This is a switch regulated by theophylline at the translation level, that is, a certain concentration of theophylline molecules in the outside world can combine with this riboswitch, and the subsequent translation process of ribosomes can be carried out through conformational changes.
 
This is a switch regulated by theophylline at the translation level, that is, a certain concentration of theophylline molecules in the outside world can combine with this riboswitch, and the subsequent translation process of ribosomes can be carried out through conformational changes.
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Revision as of 14:43, 11 October 2022


a theophylline-inducible riboswitch

This a theophylline-inducible riboswitch which works at the translation level, and the riboswitch produce low levels of background gene expression in the absence of the small molecule theophylline and large (>50-fold) increases in expression in the presence of the ligand.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]