Difference between revisions of "Part:BBa K4183003"
(→Usage and Biology) |
|||
| Line 7: | Line 7: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
We coated the engineered EcN bacteria containing BBa_K4183003 on LB agar plates containing IPTG as well as X-Gal and incubated them overnight at 37°C. The results showed that the breakdown of X-Gal could be clearly observed around the monoclonal colonies (Figure 1), which proved that lacZ was secreted extracellularly. | We coated the engineered EcN bacteria containing BBa_K4183003 on LB agar plates containing IPTG as well as X-Gal and incubated them overnight at 37°C. The results showed that the breakdown of X-Gal could be clearly observed around the monoclonal colonies (Figure 1), which proved that lacZ was secreted extracellularly. | ||
| − | [[File:HS_China_engineering-1.png|900px|thumb|none| | + | [[File:HS_China_engineering-1.png|900px|thumb|none|Figure1. Micrographs of X-Gal disassembled on LB agar plates containing X-Gal and IPTG. |
(A) When the N-terminal end of lacZ did not contain kp-sp, no disassembly of X-Gal was clearly observed around the monoclonal colonies. | (A) When the N-terminal end of lacZ did not contain kp-sp, no disassembly of X-Gal was clearly observed around the monoclonal colonies. | ||
Revision as of 14:39, 11 October 2022
Used for comparison with BBa_K4183002 to test the efficiency of secreted signal peptide.
Used for comparison with BBa_K4183002 to test the efficiency after the addition of the secreted signal peptide at the N-terminal end of lacZ.
Usage and Biology
We coated the engineered EcN bacteria containing BBa_K4183003 on LB agar plates containing IPTG as well as X-Gal and incubated them overnight at 37°C. The results showed that the breakdown of X-Gal could be clearly observed around the monoclonal colonies (Figure 1), which proved that lacZ was secreted extracellularly.
Figure1. Micrographs of X-Gal disassembled on LB agar plates containing X-Gal and IPTG. (A) When the N-terminal end of lacZ did not contain kp-sp, no disassembly of X-Gal was clearly observed around the monoclonal colonies. (B) When the N-terminal end of lacZ contains kp-sp, X-Gal is clearly observed to be disassembled around the monoclonal colony, showing a blue color.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]