Difference between revisions of "Part:BBa K4273001"

Revision as of 14:40, 11 October 2022

xyl1 Xyl1 is a xylose assimilation gene encoding xylose reductase (XR) that can converts xylose to xylitol. Xyl1 can provides an alternative carbon source for the pentose phosphate pathway that can increase S7P production. We selected Xyl1 genes from Scheffersomyces stipitis that proved to be successfully heterologous expressed for efficiently xylose-fermenting in S. cerevisiae. We used yeast toolkit (Lee, 2015) to assemble the Level 1 plasmid including the promoter, coding sequence, and terminator. Xyl1 is under the control of promoter pTDH3 which provides a high and stable transcription efficiency (Apel, et. al, 2016). Then, we used PCR amplification to obtain the DNA fragment Xyl1 from Level1 plasmid, and two homology arms (LA and RA) from yeast genome. Hello

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 89
Illegal BglII site found at 478
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 109
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K4273001 short</partinfo>
+Xyl1 is a xylose assimilation gene encoding xylose reductase (XR) that can converts xylose to xylitol. Xyl1 can provides an alternative carbon source for the pentose phosphate pathway that can increase S7P production.  We selected Xyl1 genes from Scheffersomyces stipitis that proved to be successfully heterologous expressed for efficiently xylose-fermenting in S. cerevisiae.  We used yeast toolkit (Lee, 2015) to assemble the Level 1 plasmid including the promoter, coding sequence, and terminator. Xyl1 is under the control of promoter pTDH3 which provides a high and stable transcription efficiency (Apel, et. al, 2016). Then, we used PCR amplification to obtain the DNA fragment Xyl1 from Level1 plasmid, and two homology arms (LA and RA) from yeast genome.
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