Difference between revisions of "Part:BBa K4367011:Design"

 
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===Design Notes===
 
===Design Notes===
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The exitation and emission of each flourescent protein must be apropriate to induce FRET. The litterature was searched for a good pair and the choice was validated by useing the website https://www.fpbase.org/fret/ where apropriate exitation and emission wavelengths could be found.
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This is elaborated in the long description.
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===Source===
 
===Source===

Revision as of 19:31, 11 October 2022


FRET Protein (FP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1504
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 14
    Illegal BsaI.rc site found at 1511


Design Notes

This is elaborated in the long description.

Source

It consists of two flourescent proteins, mNeongreen and mRuby3, that are fused together with a cleavable linker. The linker is a typical GS-linker and Tobacco Etch Virus Protease is able to cleave a target sequence within the linker.

References